Gene Information

Name : rpoB (GFO_2370)
Accession : YP_862401.1
Strain : Gramella forsetii KT0803
Genome accession: NC_008571
Putative virulence/resistance : Unknown
Product : DNA-directed RNA polymerase subunit beta
Function : -
COG functional category : K : Transcription
COG ID : COG0085
EC number : 2.7.7.6
Position : 2519640 - 2523452 bp
Length : 3813 bp
Strand : -
Note : DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme

DNA sequence : 
ATGTTAGCAAAGCAAACTGAAAGATTGAGTTTCTCTTCTGTTAAGAACAAGCCTGCTTATCCGGACTTTCTGGATCTGCA
GATTAAGTCTTTTCAGGACTTCTTTCAATTAGAGACAAAATCAGAAGAGCGGGGAAATGAAGGTCTTTACAACACCTTCC
TAGAAAACTTCCCCATTACGGACACCCGTAATCAATTTGTACTAGAATTCTTAGATTATTTTGTGGACCCGCCGAGATAT
TCCATTCAGGAGTGTATAGAACGCGGATTGACCTATAGTGTACCACTTAAGGCGAGACTTAAGTTATACTGTACCGACCC
TGAACACGAAGATTTTGAAACCATCGTACAGGACGTTTATTTGGGAACTATCCCTTATATGACACCTAGCGGAACTTTCT
GCATCAACGGTGCCGAGCGAGTAGTAGTTTCTCAGCTACACCGTTCTCCAGGGGTTTTCTTTGGACAGTCTTTCCATGCA
AATGGAACTAAATTATATTCTGCCCGTGTAATTCCTTTTAAAGGATCATGGATAGAATTTGCTACCGATATTAACAGCGT
AATGTACGCGTATATCGATCGTAAGAAAAAATTACCTGTTACCACGCTTTTCCGTGCTATCGGATTTGAGCGTGATAAAG
ATATTTTGGAGATCTTTGACCTTGCGGAAGAAGTGAAGGTATCTAAAACAGGACTTAAAAAATACCTTGGACGTAAACTT
GCGGCAAGAGTATTAAATACATGGTACGAAGATTTCGTAGATGAAGATACCGGAGAAGTTGTCTCTATCGAACGTAACGA
GATCGTTCTTGACCGTGATACAGAACTTGAAAAAGATCACATAGAAGAAATTCTTGAAACCGGAAGTAAAACGATTCTTC
TTCATAAAGAAGATAACCAAACCGGTGATTATGCGATCATTCATAATACACTACAAAAAGATCCTACCAACTCTGAAAAA
GAAGCGGTAGAACATATCTATCGTCAGCTTCGTAATGCTGAACCGCCAGATGAGGAAACTGCTCGTGGAATTATCGACAA
ACTTTTCTTCTCAGATCAGCGTTACAGCCTTGGAGAAGTTGGTAGATATAGAATGAACAAAAAGCTTGGTCTTGATGTTG
AAATGGATAAGCAGGTGCTTACCAAATTAGACATTATTACCATTGTTAAATATTTAATTGAACTTATCAACTCTAAAGCT
GAGATTGATGATATTGATCACCTTTCTAACCGTCGTGTAAGAACTGTAGGTGAGCAGTTATCTCAGCAGTTCGGTGTTGG
TCTTGCTCGTATGGCAAGAACAATCCGTGAGCGTATGAACGTTCGTGATAACGAAGTGTTTACTCCAATTGACTTGATCA
ACGCGAAGACATTGTCTTCTGTGATCAACTCTTTCTTCGGAACTAACCAGTTATCTCAGTTCATGGACCAGACGAACCCT
CTTGCAGAAATCACGCATAAGCGTAGACTTTCCGCATTAGGACCAGGTGGTTTATCAAGAGAAAGAGCAGGGTTCGAGGT
ACGTGATGTTCACTATACTCACTACGGAAGACTTTGTCCTATTGAAACTCCTGAAGGTCCGAATATTGGTTTGATCTCTT
CACTTTCTGTTTATGCAAAAGTGAACGGAATGGGATTCATTGAAACTCCATACCGAAGTGTAACCGATGGTAAGATCAAT
ACTTCCGAAGAGCCAATTTATTTAAGCGCTGAAGAAGAAGAAGGTAAGAAGATAGCTCAGGCTAACATTCCATTAAAAGA
TGACGGAACTATAGATACAGATCGTGTAATTGCACGTATGGAGGGTGATTTCCCGGTAGTTGATCCTAAGGAGATACATT
ATACCGATGTTGCACCAAACCAGATCTCCTCTATTTCGGCTTCATTAATTCCATTCCTGGAACATGATGATGCCAACCGT
GCATTGATGGGATCAAACATGATGCGTCAGGCAGTACCACTTTTAAGAACAGATTCTCCTATTGTTGGAACTGGACTTGA
AAGACAGGTAGCTACAGATTCTCGAGTATTGATTAATGCTGAAGGAGAAGGAGAAGTAGAATATGTAGATGCAAATAAGA
TTGTAATTAAGTACGATCGTACTGAAGAGGAAAGAATGGTAAGTTTTGATGATGATTCAAAATCTTACAACCTTATTAAA
TTCCGTAAGACGAACCAGGGATCTTGTATCAACCTGAAACCAATCATAAGTGTAGGTGACAGAGTTACTAAAGGACAGGT
TCTTTGCCAGGGTTATGCTACTGAAGCAGGTGAACTTGCATTAGGTAGAAATATGAAGGTAGCCTTCATGCCTTGGAAAG
GTTACAACTTTGAGGATGCGATTGTAATTTCAGAAAAAGTGGTAAGAGATGATATTTTCACCTCTATCCATATAGATGAA
TACTCTCTTGAAGTGAGAGATACAAAACTAGGTAACGAAGAATTGACGAATGATATTCCAAACGTTTCAGAAGAGGCTAC
TAAAGACCTTGATGAACACGGAATGATTAGAGTTGGTGCTGAAGTGAAGCCTGGTGATATTCTTATAGGTAAGATTACTC
CTAAGGGAGAAAGTGATCCAACTCCCGAAGAAAAACTTCTACGTGCGATCTTTGGTGACAAAGCAGGTGATGTAAAAGAT
GCTTCATTAAAGGCATCTCCATCATTAAGTGGTGTTGTTATTAATAAGAAGTTGTTCGCAAGAGCAATTAAAGATAAGCG
TAAGAGAGCTCAGGATAAAGAAGATGTTGCAGCACTAGAGAAAAAGTACGATGCTAAATTCGCTAATCTTAAGGCTGATC
TTGTTGAGAAACTATTCACTATTATAGGTGGAAAAACAGCTCAGGGAGTACAGAATGACCTTGGTGAAGAAGTAATGCCA
AAAGGTAAGAAGTACACGCTTAAGATGTTAAATGCTGTAGACGATTATACCCACTTAACTACGGGTACATGGACTACAGA
TGATCACCTTAATGAGCTTGTTGCAGATCTTTTACATAATTATAAAATCAAGGAAAACGATCTTCAGGGTAACCTAAGAA
GAGAGAAATTCACCGTTTCTGTTGGAGATGAACTTCCATCAGGAATTCTTAAACTGGCTAAAGTTTACATCGCTAAGAAA
CGTAAGCTGAAGGTAGGGGATAAGATGGCAGGACGTCACGGTAACAAAGGTATTGTTGCAAGAATCGTTCGTCAGGAAGA
TATGCCATTCCTTGAAGATGGAACTCCGGTTGATATCGTGTTGAACCCACTTGGGGTACCATCACGTATGAACATCGGTC
AGATCTACGAAACAGTTCTTGGATGGGCTGGTCAGAAAAACGGGAAGAAATATGCGACTCCAATTTTTGACGGTGCAACT
ATCGAAGAGATCAATGATCTTACCGATAAAGCAGGAATTCCAAGATATGGTCATACGTATCTTTATGATGGTGGAACCGG
AATGAGATTTGATCAACGTGCTACAGTTGGTGTGATTTATATGCTGAAATTAGGTCATATGATCGATGATAAGATGCACG
CCAGATCTATTGGACCATACTCACTTATTACTCAGCAACCGCTTGGTGGTAAGGCACAATTTGGTGGTCAGAGATTTGGA
GAGATGGAAGTTTGGGCATTAGAGGCTTATGGAGCTTCTGCTACCCTACGTGAAATCCTGACGGTAAAATCTGATGATGT
GATTGGTAGAGCTAAGACCTATGAGGCTATTGTGAAGGGTGAGCCAATGCCAGAACCTGGATTGCCGGAATCTTTCAATG
TACTTATGCACGAATTGAAAGGTCTTGGATTGGATATTAAATTAGAAGAATAA

Protein sequence : 
MLAKQTERLSFSSVKNKPAYPDFLDLQIKSFQDFFQLETKSEERGNEGLYNTFLENFPITDTRNQFVLEFLDYFVDPPRY
SIQECIERGLTYSVPLKARLKLYCTDPEHEDFETIVQDVYLGTIPYMTPSGTFCINGAERVVVSQLHRSPGVFFGQSFHA
NGTKLYSARVIPFKGSWIEFATDINSVMYAYIDRKKKLPVTTLFRAIGFERDKDILEIFDLAEEVKVSKTGLKKYLGRKL
AARVLNTWYEDFVDEDTGEVVSIERNEIVLDRDTELEKDHIEEILETGSKTILLHKEDNQTGDYAIIHNTLQKDPTNSEK
EAVEHIYRQLRNAEPPDEETARGIIDKLFFSDQRYSLGEVGRYRMNKKLGLDVEMDKQVLTKLDIITIVKYLIELINSKA
EIDDIDHLSNRRVRTVGEQLSQQFGVGLARMARTIRERMNVRDNEVFTPIDLINAKTLSSVINSFFGTNQLSQFMDQTNP
LAEITHKRRLSALGPGGLSRERAGFEVRDVHYTHYGRLCPIETPEGPNIGLISSLSVYAKVNGMGFIETPYRSVTDGKIN
TSEEPIYLSAEEEEGKKIAQANIPLKDDGTIDTDRVIARMEGDFPVVDPKEIHYTDVAPNQISSISASLIPFLEHDDANR
ALMGSNMMRQAVPLLRTDSPIVGTGLERQVATDSRVLINAEGEGEVEYVDANKIVIKYDRTEEERMVSFDDDSKSYNLIK
FRKTNQGSCINLKPIISVGDRVTKGQVLCQGYATEAGELALGRNMKVAFMPWKGYNFEDAIVISEKVVRDDIFTSIHIDE
YSLEVRDTKLGNEELTNDIPNVSEEATKDLDEHGMIRVGAEVKPGDILIGKITPKGESDPTPEEKLLRAIFGDKAGDVKD
ASLKASPSLSGVVINKKLFARAIKDKRKRAQDKEDVAALEKKYDAKFANLKADLVEKLFTIIGGKTAQGVQNDLGEEVMP
KGKKYTLKMLNAVDDYTHLTTGTWTTDDHLNELVADLLHNYKIKENDLQGNLRREKFTVSVGDELPSGILKLAKVYIAKK
RKLKVGDKMAGRHGNKGIVARIVRQEDMPFLEDGTPVDIVLNPLGVPSRMNIGQIYETVLGWAGQKNGKKYATPIFDGAT
IEEINDLTDKAGIPRYGHTYLYDGGTGMRFDQRATVGVIYMLKLGHMIDDKMHARSIGPYSLITQQPLGGKAQFGGQRFG
EMEVWALEAYGASATLREILTVKSDDVIGRAKTYEAIVKGEPMPEPGLPESFNVLMHELKGLGLDIKLEE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
BJAB07104_00346 YP_008207811.1 DNA-directed RNA polymerase, beta subunit/140 kD subunit Not tested AbaR25 Protein 0.0 45
BJAB0868_00350 YP_008211676.1 DNA-directed RNA polymerase, beta subunit/140 kD subunit Not tested AbaR26 Protein 0.0 45

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_009085.4918494.p0 Protein 0.0 46
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta CP000675.2.gene392.p Protein 0.0 46
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_008702.1.4609796. Protein 0.0 46
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_002695.1.914942.p Protein 0.0 46
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta CP000034.1.gene3741. Protein 0.0 46
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta CP001918.1.gene250.p Protein 0.0 46
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta CP001138.1.gene4362. Protein 0.0 46
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_010611.6233925.p0 Protein 0.0 45
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_011595.7060572.p0 Protein 0.0 45
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_010410.6003841.p0 Protein 0.0 45
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_011586.7046027.p0 Protein 0.0 45
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_010400.5987325.p0 Protein 0.0 45
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta CP004022.1.gene2794. Protein 0.0 45
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta CP000647.1.gene4402. Protein 0.0 45
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta CP002695.1.gene18.p0 Protein 0.0 44
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_002516.2.881699.p Protein 0.0 44
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_011035.1.6448762. Protein 0.0 43
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_010079.5776819.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_002951.3236234.p0 Protein 3e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_007622.3792928.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_002745.1123305.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_007795.3920377.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_009632.5315067.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_009782.5559150.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_002758.1120515.p0 Protein 2e-173 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_002953.2862188.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_002952.2860169.p0 Protein 3e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_007793.3914836.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_003923.1002608.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_013450.8613267.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_009641.5330265.p0 Protein 2e-174 42
rpoB YP_862401.1 DNA-directed RNA polymerase subunit beta NC_009487.5169226.p0 Protein 3e-173 42