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Name : lysS (Ajs_3250) Accession : YP_987449.1 Strain : Acidovorax sp. JS42 Genome accession: NC_008782 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : - Position : 3434285 - 3435832 bp Length : 1548 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGTCCACTGAAGCCACTCCTGCACCAGCCCCCCAGGACGAAAACCAACTCATCGCCGAACGCCGTGAAAAGCTCAAGGC TCTGCGCGAGAACCAGGCCCAGGGCCAAGGCGTGGCATTTCCCAATGACTACAAGCCCCAGCACCATGCCGCAGTGCTGC AGCAGCAGTACGGCACATTGGATGGAGAAGCGCTCGAAGCCCAGGCGATCACGGTGAGCGTGGCCGGCCGCATGATGCTC AAGCGCGTCATGGGCAAGGCCAGCTTCGCGACCCTGCAGGATGGATCGCTGGGTGACACCGGCGGGCGCATCCAGCTCTA CGTGACACGCGACGCCATTGGCGAGGAAGCCTATTCCGACTTCAAGCGCTGGGACCTCGGTGACATCCTGGGTGCCGAAG GCACGCTGATGAAGACCAAGACCGGCGAGCTATCGGTCAAGGTGTCGCGCCTGCGCCTGCTCACCAAGAGCCTGCGCCCC ATGCCCGACAAATTCCACGGTGTGGCCGACCAGGAGGTCAAGTACCGCCAGCGCTACGTGGACCTGATGACCGACGAGGC CGCGCGCCGCCGCTTCGTCGCGCGCAGCAAGGCTGTGGGCAGCATCCGCTCTTTCATGATGGAGCACGGCTTCCTGGAGG TGGAAACGCCCATGCTGCACCCCATTCCCGGCGGCGCCAACGCCAAGCCCTTCGTCACCCACCACAATGCGTTGGAGCAG GAGATGTTCCTGCGCATCGCGCCCGAGCTGTACCTCAAGCGCCTGATCGTCGGCGGCTTCGAGCGCGTGTTCGAGATCAA CCGCAACTTCCGCAACGAAGGCATCTCGGTGCGCCACAACCCCGAGTTCACGATGATGGAGTTTTATGCGGCCTACTGGA ACTACCGCGATCTGATGGATTTCACCGAGGCGCTGGTGCGCGATGCCGCACTCAAGGCCACGGGCACGCTGCAGCTCACC TACGGTGGCCGCGCGGTGGATCTGGCCCAGCCCTTCCAGCGCCTCACGATCCGCGAGGCCATCTACCAGCACACCGAGGC CGGCGCACATGTGGACGATGCCGCCTGGCTCATCAGCGCGCTCAAGAAACTGGGCATGACCGAGGAGAAGGACCGCCTGT CCACCCGGACCCTCGCCAGCCTGCAGGTGCTCTACTTCGAGGAAGAGGTGGAGGACAAGCTCTGGCAGCCGACCTTCATC ATGGAGCACCCGACCGAGATCAGCCCTCTGGCGCGCGCCAACGACCTGCGCCCCGAGGTGACGGAGCGCTTCGAGCTCTA CATCACCGGCCGCGAGTTCGGCAATGGCTTCTCGGAGCTGAACGATGCCGAAGACCAGGCCGCCCGCTTTCATGCGCAGG TGGCTGCCAAGGACGCGGGCGATGACGAGGCCATGTTCTTCGACCACGACTTCGTACGTGCGCTGGAATACGGCATGCCG CCCACAGGTGGTTGCGGCATCGGCATCGACCGGCTGATGATGCTGCTCACCGACAGCCCGAGCATCCGTGACGTGATCCT GTTCCCGGCCCTGCGGCGCGAGCACTGA Protein sequence : MSTEATPAPAPQDENQLIAERREKLKALRENQAQGQGVAFPNDYKPQHHAAVLQQQYGTLDGEALEAQAITVSVAGRMML KRVMGKASFATLQDGSLGDTGGRIQLYVTRDAIGEEAYSDFKRWDLGDILGAEGTLMKTKTGELSVKVSRLRLLTKSLRP MPDKFHGVADQEVKYRQRYVDLMTDEAARRRFVARSKAVGSIRSFMMEHGFLEVETPMLHPIPGGANAKPFVTHHNALEQ EMFLRIAPELYLKRLIVGGFERVFEINRNFRNEGISVRHNPEFTMMEFYAAYWNYRDLMDFTEALVRDAALKATGTLQLT YGGRAVDLAQPFQRLTIREAIYQHTEAGAHVDDAAWLISALKKLGMTEEKDRLSTRTLASLQVLYFEEEVEDKLWQPTFI MEHPTEISPLARANDLRPEVTERFELYITGREFGNGFSELNDAEDQAARFHAQVAAKDAGDDEAMFFDHDFVRALEYGMP PTGGCGIGIDRLMMLLTDSPSIRDVILFPALRREH |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 1e-134 | 55 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_987449.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 8e-135 | 55 |
