Gene Information

Name : lysS (Rcas_0009)
Accession : YP_001430166.1
Strain : Roseiflexus castenholzii DSM 13941
Genome accession: NC_009767
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 7529 - 8998 bp
Length : 1470 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGAACTCAACGAACTACAGCAACAGCGCCTTGCGAAACTCGAACGGCTGCGCGCCGCGGGCATTGATCCGTATCCGCC
ACGCGCGCAACGCACCCATACGATTGGGGAGGTCCTTGCCGGCTTCGATCAATTGATGGATCGCGGTGAACAGGTGACTG
TCGCCGGTCGGATTGTCGGTGCGCGCCGGGTGCTAGGCAAACTGGCGTTTGCCCATATCGAAGACGAGTCGGGGCGAATC
CAACTCTGGCTCAGTCGCGGCGACCTTGGCGACGAGTGGTTCGACCGGTTTCGCGACGATCTCGATACGTTCGACATTGT
CGAAGCCAGCGGAACCCTGCGCCGCACGCAACGCGGCGAACCTTCGGTGTTCGTCACGCGCATGGGAGTGCTGGCAAAGT
CGCTCAACCCGCCACCCGAAAAGTGGCACGGGCTTTCCGATATCGAGGAGCGCCATCGCCAGCGATACCTCGACCTGATC
GTCAATCCCGAGGTGCGCGACGTGTTTCGCACCCGCGCGACGATCGTCCGCACGATGCGCCGGTTTCTCGATGAACGTGG
TTTTCTCGAGGTGGAAACGCCAACCCTGCAACCGATCTACGGCGGCGCGTCTGCCCGCCCGTTCATCACCCATCACAACC
AACTGAAGCAAGATCTCTATCTACGCATCGCCGTCGAACTCTACCTGAAGCGCCTGATCGTTGGCGGTTTCGAGCGTGTC
TACGAGATCAGCAAGGTCTTCCGCAACGAAGGGGTCGATCGCACGCATAACCCTGAATTTACAATGATGGAGTGCTACCA
GGCGTATGCCGATTACCACGATATGATGCGTCTGGTGGAGGATCTGTATCGCGCGTTGGCGCTGGAAGTCACCGGAAGCA
CAACCATTGTGTTTCAGGGACAGACGATCGATTTTGGTCCTGCATGGCGGCGCGTCTCGATTCCCGCAGCGATTGCCGAA
CGTACCGGCATCGATATTCTCGAATTGACCGAACTCGAACCGCTTCAGGAGGCAATTCGCGCCGCCGGGCTGAAGGTCGA
TCTCAAGCCGGGTTGGGGGCGACAGGTCGATGAACTGTTCAGCGTCTATGTGCAACCGGCGTTGATCCAACCGACCTTCG
TGCTCGATCATCCGGTGGCGCTGTCGCCGCTGGCCAAACGACGCCCCGATCAACCTTTGCTGGTCGAACGTTTCGAGCCG
GTCGTGGCAGGAATGGAGATCGGCAATGCTTTTACTGAGTTGAACGATCCGCTCGACCAGGAACAACGCTTTCTCGAACA
GGGGCGCGCCTATGATGCTGGCGACGATGAAGCGCAACAGATGGATGTCGATTTTCTCAATGCGCTGATGTACGGCATGC
CGCCAACCGGCGGTTTGGGGATCGGCATCGACCGGACGGTCATGCTATTCACCGATCAGCCGAGCATTCGCGAGGTGATC
CTCTTCCCGCATCTGCGACCGCGGGAGTGA

Protein sequence :
MELNELQQQRLAKLERLRAAGIDPYPPRAQRTHTIGEVLAGFDQLMDRGEQVTVAGRIVGARRVLGKLAFAHIEDESGRI
QLWLSRGDLGDEWFDRFRDDLDTFDIVEASGTLRRTQRGEPSVFVTRMGVLAKSLNPPPEKWHGLSDIEERHRQRYLDLI
VNPEVRDVFRTRATIVRTMRRFLDERGFLEVETPTLQPIYGGASARPFITHHNQLKQDLYLRIAVELYLKRLIVGGFERV
YEISKVFRNEGVDRTHNPEFTMMECYQAYADYHDMMRLVEDLYRALALEVTGSTTIVFQGQTIDFGPAWRRVSIPAAIAE
RTGIDILELTELEPLQEAIRAAGLKVDLKPGWGRQVDELFSVYVQPALIQPTFVLDHPVALSPLAKRRPDQPLLVERFEP
VVAGMEIGNAFTELNDPLDQEQRFLEQGRAYDAGDDEAQQMDVDFLNALMYGMPPTGGLGIGIDRTVMLFTDQPSIREVI
LFPHLRPRE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 6e-100 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001430166.1 lysyl-tRNA synthetase VFG1668 Protein 3e-100 47