Gene Information

Name : rpoB (B21_03816)
Accession : YP_003001548.1
Strain : Escherichia coli BL21(DE3)
Genome accession: NC_012892
Putative virulence/resistance : Unknown
Product : RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.6
Position : 4089290 - 4093318 bp
Length : 4029 bp
Strand : +
Note : -

DNA sequence : 
ATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCT
CCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCC
GTTCCGTATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTT
GACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGAGCG
CGAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACG
GTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGAC
AAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGCATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGA
ATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGA
ACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAA
CTGGTGCCGGAACGCCTGCGTGGTGAAACCGCATCTTTTGACATCGAAGCTAACGGTAAAGTGTACGTAGAAAAAGGCCG
CCGTATCACTGCGCGCCACATTCGCCAGCTGGAAAAAGACGACGTCAAACTGATCGAAGTCCCGGTTGAGTACATCGCAG
GTAAAGTGGTTGCTAAAGACTATATTGATGAGTCTACCGGCGAGCTGATCTGCGCAGCGAACATGGAGCTGAGCCTGGAT
CTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATAT
CTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCG
AGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACCTGTCTGCGGTT
GGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGA
TGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCC
GTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCT
CTGGGCGATCTGGATACCCTGATGCCTCAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGG
TTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCG
GCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCA
ATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCT
TGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACT
ACGTTATCGCCCAGGCGAACTCCAACCTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGC
GAATCCAGCTTGTTCAGCCGTGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCT
GATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGC
GTGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGT
GGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGG
TATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGG
GTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATG
CGCGTAGCGTTCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCG
TTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGGCCAGAAGAGATCACCGCTGACA
TCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTATATTGGTGCGGAAGTGACCGGTGGC
GACATTCTGGTTGGTAAGGTTACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGG
TGAGAAAGCCTCTGACGTTAAAGACTCTTCTCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCT
TTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTG
TCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGA
GAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGAACAGC
TGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGAC
GATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGC
AGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGG
TAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCT
GCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCG
TGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTG
AAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAA
CTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGT
TGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGG
TTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCA
TACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAA
CATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGG
GTATCAACATCGAACTGGAAGACGAGTAA

Protein sequence : 
MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVF
DVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQME
LVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLD
LLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAV
GRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLS
LGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCP
IETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKG
ESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKR
GGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNM
RVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGG
DILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDL
SEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGD
DLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMA
AKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLK
LGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEA
YGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNVLLKEIRSLGINIELEDE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
BJAB0868_00350 YP_008211676.1 DNA-directed RNA polymerase, beta subunit/140 kD subunit Not tested AbaR26 Protein 0.0 71
BJAB07104_00346 YP_008207811.1 DNA-directed RNA polymerase, beta subunit/140 kD subunit Not tested AbaR25 Protein 0.0 71

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 NC_002695.1.914942.p Protein 0.0 100
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 CP000034.1.gene3741. Protein 0.0 99
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 CP001918.1.gene250.p Protein 0.0 99
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 CP001138.1.gene4362. Protein 0.0 99
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 CP000647.1.gene4402. Protein 0.0 99
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 CP004022.1.gene2794. Protein 0.0 94
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 NC_002516.2.881699.p Protein 0.0 74
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 NC_009085.4918494.p0 Protein 0.0 71
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 NC_010400.5987325.p0 Protein 0.0 71
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 NC_010611.6233925.p0 Protein 0.0 71
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 NC_011595.7060572.p0 Protein 0.0 71
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 NC_010410.6003841.p0 Protein 0.0 71
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 NC_011586.7046027.p0 Protein 0.0 71
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 CP000675.2.gene392.p Protein 0.0 70
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 NC_008702.1.4609796. Protein 0.0 67
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 CP002695.1.gene18.p0 Protein 0.0 65
rpoB YP_003001548.1 RNA polymerase, beta subunit, subunit of RNA polymerase, core enzyme, RNA polymerase sigma 70, RNA polymerase sigma 54, RNA polymerase sigma 32, RNA polymerase sigma 38, RNA polymerase sigma 28, RNA polymerase sigma 19 and RNA polymerase sigma 24 NC_011035.1.6448762. Protein 0.0 65