Gene Information

Name : rpoB (FIC_00280)
Accession : YP_003094810.1
Strain : Flavobacteriaceae bacterium 3519-10
Genome accession: NC_013062
Putative virulence/resistance : Unknown
Product : DNA-directed RNA polymerase subunit beta
Function : -
COG functional category : K : Transcription
COG ID : COG0085
EC number : 2.7.7.6
Position : 277474 - 281301 bp
Length : 3828 bp
Strand : +
Note : DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme

DNA sequence : 
ATGAGTAAATCTAAAGCAGTCGCTAAAACTCAGGGAACCGAAAGGATCAATTTTTCCGCCGCAAAGGGGAAGATTGAAAC
TCCTGATTTTTTGGACATTCAGATCCAATCTTTCAAAGATTTCTTCCAGCTGGATACCCTGCCGGAGCAGAGAGCAAACG
AAGCCCTCTACAAAACCTTTGAAGAAAATTTCCCGATTACAGATTCGCGGAACCAGTTTGTGCTGGAATTTCTCGATTAT
CTGGTGGATTCCCCGCGCTACTCGATCGACGAGTGTGTGGAAAGAGGACTGACGTATTCAGTTCCCCTCAAAGCCCGACT
TAAACTGTACTGTACTGACCCTGAACATGAAGATTTTCAGACCGTGGTGCAGGATGTATATTTAGGTCCGGTTCCTTATA
TGACGCCTTCAGGATCATTTATCATTAATGGTGCAGAGCGTGTTATCGTAACTCAGTTACATAGATCTCCGGGTGTGTTT
TTCGGTCAAACCTATCACGCAAACGGAACTAAACTGTATTATTCCAGAATTATCCCTTTCAAAGGATCGTGGATGGAATT
TACAACCGACATCAATAACGTAATGTACGCTTACATCGACCGTAAGAAGAAATTACCGTTAACTACATTATTAAGAGCAA
TCGGGTTCGAATCCGATAAAGATATCCTTCAGATTTTTGATCTTGCGGAAGAAGTGAAAGTTTCTAAAGCAGCATTGAAA
AAAGTAGAAGGAAGAACTTTGGCAGCGAGAGTTTTGAACACATGGTTCGAAGATTTCGTTGATGAAGACACAGGTGAAGT
AGTTTCTATCGAAAGAAACGAAATTATCCTCGACAGAGAAACCGTTCTTGAAAAAGAACATTTAGATCTGATTCTTGACG
CAGGCGTGAAATCTATTTTGATTCACAAAGAAAATTCAAACGAATTCTCTATCATCCAGAATACATTACAGAAAGATCCA
ACCAACTCCGAAAAAGAAGCGGTTGAGTATATTTACCGTCAGTTACGTAACGCAGATCCGCCAGATGAGGAAACTGCAAG
AGGAATTATTGAGAAATTATTCTTCTCCGAGCAGCGTTATTCATTAGGTGAAGTAGGCCGTTACCGTCTGAACAAGAAAT
TAGGATTAAATATCCCTGCTAAAACAGAGGTTTTAACCAAAGAAGATATCATCTCGATCGTAAGACATTTGATCGAATTG
GTGAATTCTAAAGCTGAGGTTGATGATATTGATCACTTGTCAAACAGACGTATCAAGACTGTTGGCGAGCAGCTTTCAGG
ACAGTTCGGTGTAGGTCTTTCAAGAATTGCAAGAACTATCCGTGAGCGTATGAACGTTAGAGATAACGAAATCTTTACGC
CAATTGACCTTGTTAATGCCAAGACTTTAACATCAGTAATTAACTCATTCTTTGGTACCAACCAGCTTTCTCAGTTCATG
GACCAAACCAACCCGCTATCAGAAATTACGCACAAGCGTCGTCTTTCTGCCTTGGGACCTGGTGGTTTATCAAGAGAAAG
AGCAGGTTTCGAGGTACGTGACGTTCACCATACTCACTATGGCCGAATCTGTCCGATCGAAACTCCTGAGGGACCAAACA
TTGGTTTGATTTCATCTTTAGGGCTTTATGCCAAAATCAACAGCCTTGGTTTCATTGAAACGCCTTACCGTAAAGTAACG
AACGGAAAAGTTGACCTTAAAACGACTGCAGTTTTCTTAAACGCAGAAGACGAGGAAGATAAAGTTATTGCACAGGCGAA
CGTTGAAATGAAGGAAGATGGTACCATCTCTACCGACAGAGTAATCGCCCGTTTGGATGGTGACTATCCGGTTGTAGAAC
CACAGCAGGTTGATCTGATCGATGTTGCACCTAACCAGATTTCGGGTATTTCCGCTTCATTAATTCCATTCCTGGAGCAT
GATGATGCGAACCGTGCGCTGATGGGATCCAACATGATGCGTCAGGCAGTTCCGTTGTTGAAACCTGAAGCACCAATCGT
AGGTACAGGACTTGAGCAGCAGGTTGCAAGAGATTCAAGAATCTTGATCAACGCTGAAGGCAATGGTACTGTAGAATATG
TTGATGCAGACAAGATCACAATTAAATATGAAAGAACAGAAGACGATGATTTAGTATCATTCGAATCTGCTACCAAGACG
TATAAATTAACCAAATTCCGCAAAACCAACCAAAGTACAACGATCACTTTGAGACCAAACGTAAGAGTAGGCGACAAAGT
TCAGAAAGGTCAGGTACTTTGCGACGGTTATGCAACAGAAAATGGTGAACTGGCATTGGGTAGAAACCTTGTGGTAGCCT
TCATGCCATGGAAAGGGTACAACTTTGAGGATGCGATCGTAATCAACGAAAAAGTTGTGCGCGAAGACTGGTTTACTTCC
ATCCACGTTGATGAGTATTCGCTTGAAGTTCGTGATACCAAATTAGGTATGGAAGAACTGACAGCAGATATCCCTAACGT
TTCTGAAGAGGCTACAAAAGACCTTGACGAAAACGGTATGATCAGAATTGGAGCCGACGTAAAACCTGGCGACATTATGA
TTGGTAAGATTACTCCAAAAGGAGAATCAGATCCAACTCCTGAAGAGAAACTTCTTCGTGCGATCTTCGGTGATAAAGCC
GGCGACGTGAAGGATGCTTCTTTAAAGGCAGATTCTTCGTTAAGAGGTGTTGTAATCAATAAAAAACTCTTCTCAAGAAA
CATCAAAGACAAAAAGAAAAGAACCGAAGAGAAACTTAAACTTGAGGAAATCGAAAACACGTATAAAGCGAAATTCGATC
AGTTAAGAAACACACTTCTCGAAAAACTCGGTACGCTTGTTAACGGTAAAACTTCTCAGGGTGTGAACAACGACCTTGAT
GAGGAAATCATCGGAAAAGGCGTGAAGTTTACAACGAAATTACTTCAGAGTGTTGAAGATTACGTGAACGTAAGCGGAGC
TGACTGGACTGTTGATGCCGATAAAAACGAACTGATCAAGCAGTTGATCCACAACTATAAGATTAAGTTCAACGATATTC
AGGGTGTTAAAAACCGTGAGAAATTCGCAATTTCAATCGGTGATGAACTTCCTGCAGGTATCATTAAACTGGCTAAAGTA
TATGTTGCTAAGAAACGTAAACTTAATGTTGGTGATAAGATGGCGGGACGTCACGGTAACAAAGGTATTGTATCAAGAAT
CGTTCGCGAGGAAGATATGCCTTTCCTTGAAGACGGAACACCGGTAGATATCGTACTGAACCCGCTGGGTGTACCTTCGC
GTATGAACATCGGTCAGATTTACGAAACTGTACTTGGATGGGCTGGTCAGAAATTAGGTTTGAAGTTTGCGACGCCGATC
TTCGATGGTGCAAATATCGACCAGATTACAGGCTATACCGAGCAGGCAGGTCTTCCGGAATTCGGATCGACTTATCTTTA
TGATGGTGGTACCGGCGAAAGATTCTCACAACCGGCAACAGTTGGGGTAATTTATATGCTGAAACTGGGTCACATGGTTG
ATGACAAAATGCACGCACGTTCAATTGGGCCATATTCATTGATCACGCAGCAGCCACTTGGTGGTAAAGCGCAGTTCGGT
GGACAGCGTTTCGGTGAGATGGAGGTTTGGGCGCTTGAGGCATTTGGTGCTTCTAACATCCTTAGAGAAATCCTTACCGT
GAAATCCGATGACGTAATTGGTAGAGCGAAAACTTACGAAGCCATCGCGAAAGGCGAAGCAATGCCCGAACCGGGTATTC
CTGAATCTTTCAACGTGTTGCTTCACGAGCTACAGGGCCTTGGCCTTGACGTAAGACTAGAGGAATAA

Protein sequence : 
MSKSKAVAKTQGTERINFSAAKGKIETPDFLDIQIQSFKDFFQLDTLPEQRANEALYKTFEENFPITDSRNQFVLEFLDY
LVDSPRYSIDECVERGLTYSVPLKARLKLYCTDPEHEDFQTVVQDVYLGPVPYMTPSGSFIINGAERVIVTQLHRSPGVF
FGQTYHANGTKLYYSRIIPFKGSWMEFTTDINNVMYAYIDRKKKLPLTTLLRAIGFESDKDILQIFDLAEEVKVSKAALK
KVEGRTLAARVLNTWFEDFVDEDTGEVVSIERNEIILDRETVLEKEHLDLILDAGVKSILIHKENSNEFSIIQNTLQKDP
TNSEKEAVEYIYRQLRNADPPDEETARGIIEKLFFSEQRYSLGEVGRYRLNKKLGLNIPAKTEVLTKEDIISIVRHLIEL
VNSKAEVDDIDHLSNRRIKTVGEQLSGQFGVGLSRIARTIRERMNVRDNEIFTPIDLVNAKTLTSVINSFFGTNQLSQFM
DQTNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHHTHYGRICPIETPEGPNIGLISSLGLYAKINSLGFIETPYRKVT
NGKVDLKTTAVFLNAEDEEDKVIAQANVEMKEDGTISTDRVIARLDGDYPVVEPQQVDLIDVAPNQISGISASLIPFLEH
DDANRALMGSNMMRQAVPLLKPEAPIVGTGLEQQVARDSRILINAEGNGTVEYVDADKITIKYERTEDDDLVSFESATKT
YKLTKFRKTNQSTTITLRPNVRVGDKVQKGQVLCDGYATENGELALGRNLVVAFMPWKGYNFEDAIVINEKVVREDWFTS
IHVDEYSLEVRDTKLGMEELTADIPNVSEEATKDLDENGMIRIGADVKPGDIMIGKITPKGESDPTPEEKLLRAIFGDKA
GDVKDASLKADSSLRGVVINKKLFSRNIKDKKKRTEEKLKLEEIENTYKAKFDQLRNTLLEKLGTLVNGKTSQGVNNDLD
EEIIGKGVKFTTKLLQSVEDYVNVSGADWTVDADKNELIKQLIHNYKIKFNDIQGVKNREKFAISIGDELPAGIIKLAKV
YVAKKRKLNVGDKMAGRHGNKGIVSRIVREEDMPFLEDGTPVDIVLNPLGVPSRMNIGQIYETVLGWAGQKLGLKFATPI
FDGANIDQITGYTEQAGLPEFGSTYLYDGGTGERFSQPATVGVIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFG
GQRFGEMEVWALEAFGASNILREILTVKSDDVIGRAKTYEAIAKGEAMPEPGIPESFNVLLHELQGLGLDVRLEE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
BJAB07104_00346 YP_008207811.1 DNA-directed RNA polymerase, beta subunit/140 kD subunit Not tested AbaR25 Protein 0.0 44
BJAB0868_00350 YP_008211676.1 DNA-directed RNA polymerase, beta subunit/140 kD subunit Not tested AbaR26 Protein 0.0 44

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_009085.4918494.p0 Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_010400.5987325.p0 Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_010611.6233925.p0 Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_011595.7060572.p0 Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_010410.6003841.p0 Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_011586.7046027.p0 Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta CP001138.1.gene4362. Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_002695.1.914942.p Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta CP000034.1.gene3741. Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta CP001918.1.gene250.p Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta CP000647.1.gene4402. Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_008702.1.4609796. Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta CP002695.1.gene18.p0 Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_011035.1.6448762. Protein 0.0 44
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta CP000675.2.gene392.p Protein 0.0 43
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta CP004022.1.gene2794. Protein 0.0 43
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_002516.2.881699.p Protein 0.0 42
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_009782.5559150.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_002951.3236234.p0 Protein 2e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_002953.2862188.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_013450.8613267.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_007793.3914836.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_003923.1002608.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_009641.5330265.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_002758.1120515.p0 Protein 8e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_010079.5776819.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_002952.2860169.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_007622.3792928.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_002745.1123305.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_007795.3920377.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_009632.5315067.p0 Protein 1e-162 41
rpoB YP_003094810.1 DNA-directed RNA polymerase subunit beta NC_009487.5169226.p0 Protein 1e-161 41