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PAthogenisity Island DataBase (PAI DB)

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Homologs of unnamed gene of AY147025

protein-level

Hit (GenBank Accn)

Product

Source Genome

Eval.

Ident.(%)

1) hopX1
( YP_273555.1 )

type III effector HopX1

NC_005773
(Pseudomonas syringae 1448A)

4e-146

2) Psyr_1220
( YP_234312.1 )

type III effector HopX1

NC_007005
(Pseudomonas syringae B728a)

3e-126

3) HopX1
( NP_808672.1 )

type III effector HopX1

NC_004633
(Pseudomonas syringae DC3000)

1e-109

• Sequence Alignments

1) hopX1 in NC_005773


> AY147025|4|G3|32419323|AAN85175.1|Pseudomonas_syringae_pv._phaseolicola_BK378|-|934..2256|-|-|AvrPphE8
Length=440

 Score =  510 bits (1924),  Expect = 4e-146, Method: Compositional matrix adjust.
 Identities = 373/374 (99%), Positives = 373/374 (99%), Gaps = 0/374 (0%)

Query  1    MRIHSAGHSLPAPGPSVETTEKAVqsssaqnpascssqTERPEAGSTQVRPNYPYSSVKT  60
            MRIHSAGHSLPAPGPSVETTEKAVQSSSAQNPASCSSQTERPEAGSTQVRPNYPYSSVKT
Sbjct  1    MRIHSAGHSLPAPGPSVETTEKAVQSSSAQNPASCSSQTERPEAGSTQVRPNYPYSSVKT  60

Query  61   RLPPVSSTGQAISDTpsslpgylllrrldrrpldedsIKALVPADealrearralPFGRG  120
            RLPPVSSTGQAISDTPSSLPGYLLLRRLDRRPLDEDSIKALVPADEALREARRALPFGRG
Sbjct  61   RLPPVSSTGQAISDTPSSLPGYLLLRRLDRRPLDEDSIKALVPADEALREARRALPFGRG  120

Query  121  NIDVDAQRTHLQSGARAVAAKRLRKDAERAGHEPMPENDEMNWHVLVAMSGQVFGAGNCG  180
            NIDVDAQRTHLQSGARAVAAKRLRKDAERAGHEPMPENDEMNWHVLVAMSGQVFGAGNCG
Sbjct  121  NIDVDAQRTHLQSGARAVAAKRLRKDAERAGHEPMPENDEMNWHVLVAMSGQVFGAGNCG  180

Query  181  EHARIASFAYGALAQESGRSPREKIHLAEQPGKDHVWAETDNSSAGSSPIVMDPWSNGAA  240
            EHARIASFAYGALAQESGRSPREKIHLAEQPGKDHVWAETDNSSAGSSPIVMDPWSNGAA
Sbjct  181  EHARIASFAYGALAQESGRSPREKIHLAEQPGKDHVWAETDNSSAGSSPIVMDPWSNGAA  240

Query  241  ILAEDSRFAKDRSAVERTYSFTLAMAAEAGKVARETAENVLTHTTSRLQKRLADQLPNVS  300
            ILAEDSRFAKDRSAVERTYSFTLAMAAEAGKVARETAENVLTHTTSRLQKRLADQLPNVS
Sbjct  241  ILAEDSRFAKDRSAVERTYSFTLAMAAEAGKVARETAENVLTHTTSRLQKRLADQLPNVS  300

Query  301  PLEGGRYQPKKSVLDEAFARRVSDKLNSDDPRRALQMEIEAVGVAMSLGAEGVKTVARQA  360
            PLEGGRYQP KSVLDEAFARRVSDKLNSDDPRRALQMEIEAVGVAMSLGAEGVKTVARQA
Sbjct  301  PLEGGRYQPEKSVLDEAFARRVSDKLNSDDPRRALQMEIEAVGVAMSLGAEGVKTVARQA  360

Query  361  PKVVRQARSVASSK  374
            PKVVRQARSVASSK
Sbjct  361  PKVVRQARSVASSK  374

2) Psyr_1220 in NC_007005


> AY147025|4|G3|32419323|AAN85175.1|Pseudomonas_syringae_pv._phaseolicola_BK378|-|934..2256|-|-|AvrPphE8
Length=440

 Score =  444 bits (1673),  Expect = 3e-126, Method: Compositional matrix adjust.
 Identities = 292/375 (78%), Positives = 346/375 (93%), Gaps = 1/375 (0%)

Query  1    MRIHSSGHGISGPVSSAETVEKAVQSSAQAQNEASHSGPSEHPESRSCQARPNYPYSSVK  60
            MRIHS+GH++++P +S ET EKAVQSS+ AQN AS S+ +E+PE+ S Q RPNYPYSSVK
Sbjct  1    MRIHSAGHSLPAPGPSVETTEKAVQSSS-AQNPASCSSQTERPEAGSTQVRPNYPYSSVK  59

Query  61   TRLPPVASAGQSLSETPSSLPGYlllrrldrrpldQDAIKGLIPADEAVGEARRALPFGR  120
            TRLPPV+S+GQ++S+TPSSLPGYLLLRRLDRRPLD+D+IK+L+PADEA+ EARRALPFGR
Sbjct  60   TRLPPVSSTGQAISDTPSSLPGYLLLRRLDRRPLDEDSIKALVPADEALREARRALPFGR  119

Query  121  GNIDVDAQRSNLESGARTLAARRLRKDAETAGHEPMPENEDMNWHVLVAMSGQVFGAGNC  180
            GNIDVDAQR++L+SGAR++AA+RLRKDAE AGHEPMPEN++MNWHVLVAMSGQVFGAGNC
Sbjct  120  GNIDVDAQRTHLQSGARAVAAKRLRKDAERAGHEPMPENDEMNWHVLVAMSGQVFGAGNC  179

Query  181  GEHARIASFAYGASAQEKGRAGDENIHLAAQSGEDHVWAETDDSSAGSSPIVMDPWSNGP  240
            GEHARIASFAYGA AQE GR+  E+IHLA Q+G DHVWAETD+SSAGSSPIVMDPWSNG+
Sbjct  180  GEHARIASFAYGALAQESGRSPREKIHLAEQPGKDHVWAETDNSSAGSSPIVMDPWSNGA  239

Query  241  AVFAEDSRFAKDRRAVERTDSFTLSTAAKAGKITRETAEKALTQATSrlqqrladqqaqV  300
            A++AEDSRFAKDR AVERT SFTL+ AA AGK++RETAE+ LT++TSRLQ+RLADQ ++V
Sbjct  240  AILAEDSRFAKDRSAVERTYSFTLAMAAEAGKVARETAENVLTHTTSRLQKRLADQLPNV  299

Query  301  SPVEGGRYRQENSVLDDAFARRVSDMLNNADPRRALQVEIEASGVAMSLGAQGVKTVVRQ  360
            SP+EGGRY+ E+SVLD+AFARRVSD LN+ DPRRALQ+EIEA GVAMSLGA+GVKTV RQ
Sbjct  300  SPLEGGRYQPEKSVLDEAFARRVSDKLNSDDPRRALQMEIEAVGVAMSLGAEGVKTVARQ  359

Query  361  APKVVRQARGVASAK  375
            APKVVRQAR+VAS+K
Sbjct  360  APKVVRQARSVASSK  374

3) HopX1 in NC_004633


> AY147025|4|G3|32419323|AAN85175.1|Pseudomonas_syringae_pv._phaseolicola_BK378|-|934..2256|-|-|AvrPphE8
Length=440

 Score =  389 bits (1464),  Expect = 1e-109, Method: Compositional matrix adjust.
 Identities = 255/382 (67%), Positives = 318/382 (84%), Gaps = 4/382 (1%)

Query  1    MKIHNAGLTPPLPGISNGNVGKAAQSSITQPQSQQGSYGLPPESSETRPDRARANYPYSS  60
            M+IH+AG + P PG S     KA QSS +Q   + +S +   E +E+ + + R+NYPYSS
Sbjct  1    MRIHSAGHSLPAPGPSVETTEKAVQSSSAQ---NPASCSSQTERPEAGSTQVRPNYPYSS  57

Query  61   VQTRLPPVASAGKPLPDTPSSLPGYLLLRRLDHRPVDQEGTKSLIPADKAVAEARRALPF  120
            V+TRLPPV+S+G++++DTPSSLPGYLLLRRLD+RP+D+++ K+L+PAD A+ EARRALPF
Sbjct  58   VKTRLPPVSSTGQAISDTPSSLPGYLLLRRLDRRPLDEDSIKALVPADEALREARRALPF  117

Query  121  GRGNIDVDAQLSNLESGARTLAARCLRKDAEAAGHEPMPANEPMNWHVLVAMSGQVFGAG  180
            GRGNIDVDAQ ++L+SGAR++AA+ LRKDAE AGHEPMP N+ MNWHVLVAMSGQVFGAG
Sbjct  118  GRGNIDVDAQRTHLQSGARAVAAKRLRKDAERAGHEPMPENDEMNWHVLVAMSGQVFGAG  177

Query  181  NCGEHARIASFAYGALAQENGRSEYENIYLAASTEEDHVWAETDESQSGTSTIVMDPWSN  240
            NCGEHARIASFAYGALAQE+GRS  E+I LA     DHVWAETD+S +G+S IVMDPWSN
Sbjct  178  NCGEHARIASFAYGALAQESGRSPREKIHLAEQPGKDHVWAETDNSSAGSSPIVMDPWSN  237

Query  241  GSAIFAEDSRFAKNRNAVERTDTFNLSTAAEAGKITRETAEKALTQVTTRLQKRLADQQE  300
            G+AI+AEDSRFAK+R+AVERT +F L+ AAEAGK++RETAE+ LT+ T+RLQKRLADQ  
Sbjct  238  GAAILAEDSRFAKDRSAVERTYSFTLAMAAEAGKVARETAENVLTHTTSRLQKRLADQLP  297

Query  301  QVSPIKSGRYRPEKSVLDDAFVRRVSDKLTSPDLRRALQVDIEAVGVAMSLGTKGVKDAT  360
            +VSP+ +GRY+PEKSVLD+AF RRVSDKL S D RRALQ++IEAVGVAMSLG+ GVK  +
Sbjct  298  NVSPLEGGRYQPEKSVLDEAFARRVSDKLNSDDPRRALQMEIEAVGVAMSLGAEGVKTVA  357

Query  361  RQARPLVELAVKVASP-QGLAR  381
            RQA  +V  A  VAS+ +GL +
Sbjct  358  RQAPKVVRQARSVASSKEGLKK  379