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PAthogenisity Island DataBase (PAI DB)

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Homologs of unnamed gene of AY147028

protein-level

Hit (GenBank Accn)

Product

Source Genome

Eval.

Ident.(%)

1) AvrB3
( YP_234311.2 )

type III effector protein AvrB3

NC_007005
(Pseudomonas syringae B728a)

2e-109

2) avrB4-1
( YP_275207.1 )

type III effector AvrB4-1

NC_005773
(Pseudomonas syringae 1448A)

2e-72

3) avrB4-2
( YP_273068.1 )

type III effector AvrB4-2

NC_005773
(Pseudomonas syringae 1448A)

3e-72

• Sequence Alignments

1) AvrB3 in NC_007005


> AY147028|6|G5|32419358|AAN85189.1|Pseudomonas_syringae_pv._syringae_W4N_15|-|3102..4088|-|-|unknown
Length=328

 Score =  388 bits (1458),  Expect = 2e-109, Method: Compositional matrix adjust.
 Identities = 246/328 (75%), Positives = 287/328 (88%), Gaps = 5/328 (1%)

Query  1    MGCVsskasvissdsFRASYTNSP-----EASSVHQRARTPRCGELQGPQVSRLMPYQQA  55
            MGCVSSKASVISSDSFRASYTNSP     E+SSVH+RARTPR GELQGPQ SRLMPYQQA
Sbjct  1    MGCVSSKASVISSDSFRASYTNSPGAFRAESSSVHNRARTPRYGELQGPQASRLMPYQQA  60

Query  56   LVGVARWPNPHFNRDDAPHQMEYGESFYHKSRELGASVANGEIETFQELWSEARDWRASR  115
            L+GVARWP++HFNRDDAPHQMEYG+SFY+KSR LG+SVANGEI +FQELW EARDWRASR
Sbjct  61   LIGVARWPDSHFNRDDAPHQMEYGQSFYNKSRALGGSVANGEIGSFQELWDEARDWRASR  120

Query  116  AGQDARLFSSSRDPNSSRAFVTPITGPYEFLKDRFANRKDGEKHKMLDFLPHSNTFRFHG  175
            AG DA +F S+RDPNS R + TP++  Y ++KDRFANRKDGE     DFLP S+TFR+ G
Sbjct  121  AGADADVFLSPRDPNSYREYATPLAEQYSYIKDRFANRKDGEVGSPADFLPKSKTFRISG  180

Query  176  KIDGERLPLTWISISSDRRADRTKDPYQRLRDQGMNDVGEPNVMLHTQAEYVPKIMQHVE  235
            KIDGE++PLT+I++S DR+ADR  DPY RLR+QG  D+GEPN+M HT AEYVP+IM HVE
Sbjct  181  KIDGEQIPLTRITVSKDRHADRMADPYPRLRNQGHGDLGEPNLMSHTSAEYVPQIMSHVE  240

Query  236  HLYKaatdaalsdanalKKLAEIHWWTVQAVPDFRGSAAKAELCVRSIAQARGMDLPPMR  295
             L ++ATD+++SD++ALK LAE+HWW  +A+PD RGSAAK+ELCVRSIAQARGMDLPPMR
Sbjct  241  SLHQSATDPSVSDSHALKTLAEMHWWMAHAMPDKRGSAAKTELCVRSIAQARGMDLPPMR  300

Query  296  LGIVPDLEALTMPLKDFVKSYEGFFEHN  323
            LGIVPDLEALTMPLKDFVKSY+GFFE+N
Sbjct  301  LGIVPDLEALTMPLKDFVKSYQGFFENN  328

2) avrB4-1 in NC_005773


> AY147028|6|G5|32419358|AAN85189.1|Pseudomonas_syringae_pv._syringae_W4N_15|-|3102..4088|-|-|unknown
Length=328

 Score =  265 bits (991),  Expect = 2e-72, Method: Compositional matrix adjust.
 Identities = 158/332 (48%), Positives = 237/332 (72%), Gaps = 14/332 (4%)

Query  1    MGCITSKPLVSSPQW------HNSATNSENLETGQRSHKASLYGAMSGSPERSELTWHQQ  54
            MGC++SK+ V S++       +++++   +  + +   +++ YG + G P+ S L   QQ
Sbjct  1    MGCVSSKASVISSDSFRASYTNSPGAFRAESSSVHNRARTPRYGELQG-PQASRLMPYQQ  59

Query  55   SLVGVARWPDKEYNRDHVPLQMEYGRSFWNESRKIGSALANGEIQNFEDLWEKARDWRCS  114
            +L+GVARWPD ++NRD+ P QMEYG+SF N SR +G+++ANGEI +F++LW+ ARDWR S
Sbjct  60   ALIGVARWPDSHFNRDDAPHQMEYGQSFYNKSRALGGSVANGEIGSFQELWDEARDWRAS  119

Query  115  MANHDENIFKKPR--NSYNEFPFTTPLINQYNYIKERYSARTDGSLQKLDDEGLLPPAKE  172
             A  D ++F  PR  NSY E+  +TPL +QY+YIK+R++ R DG +    D  +LP +K 
Sbjct  120  RAGADADVFLSPRDPNSYREY--ATPLAEQYSYIKDRFANRKDGEVGSPAD--FLPKSKT  175

Query  173  FLITDKIFGEPISLTKIVCSSDSSAHRDQRRYSDLWSRGL-DYGEPHYIQHTSSEEVPKI  231
            F I++KI GE I+LT+I  S D  A+R    Y+ L+++G  D GEP+ + HTS+E VP+I
Sbjct  176  FRISGKIDGEQIPLTRITVSKDRHADRMADPYPRLRNQGHGDLGEPNLMSHTSAEYVPQI  235

Query  232  LHHVNDLFNEVLQSNLSTKKALKFLGEIHWWLAHAMPDERGSAAKSELCVRAIAQAKGLD  291
            + HV+ L +   ++++S   ALK L+E+HWW+AHAMPD RGSAAK+ELCVR+IAQA+G+D
Sbjct  236  MSHVESLHQSATDPSVSDSHALKTLAEMHWWMAHAMPDKRGSAAKTELCVRSIAQARGMD  295

Query  292  LPPMKSGIVPDLEAMTMSREQFIKQYPSMFDD  323
            LPPM+ GIVPDLEA+TM+  +F+K Y + F++
Sbjct  296  LPPMRLGIVPDLEALTMPLKDFVKSYQGFFEN  327

3) avrB4-2 in NC_005773


> AY147028|6|G5|32419358|AAN85189.1|Pseudomonas_syringae_pv._syringae_W4N_15|-|3102..4088|-|-|unknown
Length=328

 Score =  265 bits (989),  Expect = 3e-72, Method: Compositional matrix adjust.
 Identities = 162/332 (49%), Positives = 237/332 (72%), Gaps = 14/332 (4%)

Query  1    MGCITSKP-LVSSPRWHNSATNS-----ENLETGQRSHKASLYGAISGSPERSELTWHQQ  54
            MGC++SK+ ++SS   + S TNS      +  + +   +++ YG + G P+ S L   QQ
Sbjct  1    MGCVSSKASVISSDSFRASYTNSPGAFRAESSSVHNRARTPRYGELQG-PQASRLMPYQQ  59

Query  55   SLVGVARWPDKEYNRDHVPLQMEYGRSFWNESRKIGSALANGEIQNFEDLWEKARDWRCS  114
            +L+GVARWPD ++NRD+ P QMEYG+SF N SR +G+++ANGEI +F++LW+ ARDWR S
Sbjct  60   ALIGVARWPDSHFNRDDAPHQMEYGQSFYNKSRALGGSVANGEIGSFQELWDEARDWRAS  119

Query  115  MANHDENIFKKPR--NSYNEFPFTTPLINQYNYIKERYSARTDGSLQKLDDEGLLPPAKE  172
             A  D ++F  PR  NSY E+  +TPL +QY+YIK+R++ R DG +    D  +LP +K 
Sbjct  120  RAGADADVFLSPRDPNSYREY--ATPLAEQYSYIKDRFANRKDGEVGSPAD--FLPKSKT  175

Query  173  FLITDKIFGEPISLTKIVCSSDSSAHRDQRRYSDLWSRGL-DYGEPHYIQHTSSEEVPKI  231
            F I++KI GE I+LT+I  S D  A+R    Y+ L+++G  D GEP+ + HTS+E VP+I
Sbjct  176  FRISGKIDGEQIPLTRITVSKDRHADRMADPYPRLRNQGHGDLGEPNLMSHTSAEYVPQI  235

Query  232  LHHVNDLFDEVLQSNLSTKKALKFLGEIHWWLAHAMPDERGSAAKSELCVRAIAQAKGLD  291
            + HV+ L +   ++++S   ALK L+E+HWW+AHAMPD RGSAAK+ELCVR+IAQA+G+D
Sbjct  236  MSHVESLHQSATDPSVSDSHALKTLAEMHWWMAHAMPDKRGSAAKTELCVRSIAQARGMD  295

Query  292  LPPMKSGIVPDLEAMTMSREQFIKQYPSMFDD  323
            LPPM+ GIVPDLEA+TM+  +F+K Y + F++
Sbjct  296  LPPMRLGIVPDLEALTMPLKDFVKSYQGFFEN  327